Growing Lion's Mane
On Masters' Mix

Learn How To Grow Lion's Mane On Supplemented Hardwood

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What Is Lion's Mane Mushroom?

Lion’s mane (hericium erinaceus) is a hardwood loving fungi that is considered to perform at it its best when grown on masters’ mix. Master’s mix is a highly supplemented hardwood substrate mix which is sterilised before being inoculated with lion’s mane grain spawn following aseptic technique. 

In this guide we demonstrate the preparation of the substrate, inoculating the substrate with grain spawn, incubation of the fruiting block and how to fruit and harvest the final product.

Health Benefits Of Lion's Mane

Lion’s mane is being investigated because of it’s potential to treat a number of conditions primarily involving neuroregeneration. Studies have shown it to be effective in treating the following.

  • Dementia
  • Alzheimer’s disease
  • Ischemic Stroke
  • Parkinson’s disease
  • Protection against depressive symptoms
  • Neuropathic pain
  • Presbycusis
  • Learn more here

What You'll Need

In this tutorial we will be listing the quantities to produce 1 2.5 kilogram fruiting block. The substrate should weigh 2.3 kilograms with an additional 200 grams of grain spawn. 

Step 1. Prepare Substrate

Using kitchen scales weigh 450 grams of each hardwood fuel pellets & soy hulls and add to a filter patch grow bag. Weigh 1.4 kg (L) of water and add to the dry ingredients.

Moisture content in large batches can vary greatly if not thoroughly mixed just prior to packing. For this reason it’s recommended to weigh and pack each bag individually to ensure that the moisture content remains consistent in each bag.

Step 2. Fold Grow Bags For Sterilisation

The grow bags must be folded for sterilisation. Filter patch grow bags are self sealing under pressure and do not need to be sealed before sterilising when folded correctly.

Learn how we fold mushroom grow bags in the link below.

Step 3. Sterilise Grow Bags

The folded grow bags packed with substrate must be loaded into the pressure cooker and sterilised for a minimum of 90 minutes at 15PSI.

Allow the pressure cooker to depressurise without intervention. Do not open until cool.


23 qt presto

Can I Use An Instapot?

It’s not essential to have access to a stove-top pressure cooker or canner. An instapot or electric bench-top pressure cooker can be used instead. 

An instapot will often only reach approximately 7 – 8 PSI and for this reason sterilisation times for preparing grain spawn may need to be extended by 30 minutes.

A typical instapot will fit 4 spawning jars per cycle.

Step 4. Inoculate With Grain Spawn

Once the grow bag has cooled down it can inoculated with grain spawn. We will be adding approximately 200 grams of grain spawn per 2.3 kg grow bag. 

Lion’s mane grain spawn is very fine and can sometimes appear immature after being shaken. Despite appearances each individual grain remains colonised and viable.

It’s important to inoculate each grow bag while adhering to aseptic technique. 

Well Colonised Lion's Mane Grain Spawn

Lion's Mane Spawn

Fully Colonised Lion's Mane Grain Spawn After Shaking

Lion's Mane Spawn After Shaking

Aseptic Technique

It’s important to adhere to aseptic technique when inoculating sterile media. Sterilised media is extremely prone to contamination.

Only objects that have been flame sterilised may make contact with the sterile media or grain spawn.

Alcohol can be used to sanatise and clean surfaces of micro-particles but is not suitable for sterilisation.

A face mask should be worn at all times. All work should be undertaken with a laminar flow hood when possible. In the absence of a flow hood a smaller enclosed space with minimal airflow, such as a bathroom may be used with varied results.

Step 5. Seal The Grow Bag & Wait

The inoculated filter patch grow bag needs to be sealed immediately after inoculation to prevent the entrance of competing pathogens like yeast & mould. An impulse sealer can be used to quickly and securely seal grow bags. Alternatively zip-ties or grow bag clips can be used.

The fruiting block can be stored at room temperature & out of direct sunlight for 14 – 21 days or until fully colonised.

Colonised Base Of Lion's Mane Fruiting Block

Visibly White Primordia Forming On Lion's Mane Fruiting Block

When Is Lion's Mane Fully Colonised?

Lion’s mane mycelium is very fine in comparison to other commonly grown gourmet mushrooms like oyster mushrooms. It can sometimes be difficult to identify when lion’s mane is fully colonised. 

The grow kit will begin to feel firmer and slightly lighten in colour as it colonises. The mycelium will remain largely transparent with large amounts of dark substrate showing through, this is often in relation to the concentration gradient of supplementation in the bulk substrate.

The lion’s mane mycelium will only become visibly white as it begins to knot and form primordia. This will tend to occur where the substrate makes most contact with fresh air.

Step 6. Opening The Grow Bag

The lion’s mane fruiting block can be opened once fully colonised. As much air as possible should be removed from the grow bag upon opening. The grow bag can be folded tight against the fruiting block and taped in place. 

We recommend making a small incision no longer than 5 cm for fruiting. Primordia may form in the humid space between the bag and substrate however it will abort due to a lack of space and fresh air exchange. This will ensure that the nutrition is spent forming a larger fruiting body.

For first time growers it can be easiest to allow primordia to form within the bag prior to opening to expose the candidate to the required fresh air and humidity.

X Shaped Cut On Lion's Mane Fruiting Block

5 cm Cut On Top Of Lion's Mane Fruiting Block

Step 7. Store In Fruiting Chamber

Now it’s time to place your lion’s mane in a greenhouse or fruiting chamber. It’s common practice to use a mini-greenhouse or large plastic crate to assist in creating a humid micro-climate. If you’re short on space we recommend making a shotgun fruiting chamber (SGFC) using a plastic crate and perlite.

Mist the walls of your greenhouse at least twice daily to increase humidity. If the mushroom appears to be growing long and leggy try increasing the amount of fresh air it receives. Yellowing is usually the result of low humidity.

Using A Humidifier

Keeping mushrooms humid is key to ensuring consistent yields. A humidifier will ensure that the fruiting chamber has ample humidity to ensure consistently impressive yields. Learn more about maintaining humidity in our online guide.

Step 8. Harvest & Enjoy

The mushroom is considered fully mature when it stops growing larger and the spines grow longer. This may take between 2 – 3 weeks. It can be picked at different stages of maturity for a different texture. Younger lion’s mane mushrooms have a firmer and denser texture.

The fibrous structure of lion’s mane makes it the perfect candidate as a meat-like textured substitute in cooking. It can be cooked as steaks, in soups or even battered and fried. 

Lion's Mane Grain Spawn

Grain spawn is a little known secret weapon of the world of mushroom cultivation. It’s made from sterilised grains that have been inoculated with a live mycelium culture.

Grain spawn contains a lot of energy for the mycelium to consume. It’s a bit like rocket fuel for mushrooms. Once it’s added to your substrate it takes off like wild fire. 

Mother Nature works her magic using spores but in a controlled environment you need to tip the scales in your favour by using clean high-quality grain spawn so you are doing your best to ensure success. 

Well Colonised Lion's Mane Grain Spawn
Lion's Mane Mushroom Pakora Vegan Recipe

Lion's Mane Ready To Grow Kit

Learn to grow your own mushrooms with one of our ready to fruit mushroom grow kits. 

Our kits consist of supplement hardwood which has been inoculated under laboratory conditions with a live mycelium culture.

Learn more about using a mushroom grow kit with our Step By Step Guide.

View Our Range Of Grain Spawn

Who We Are

We’re a small scale producer of gourmet mushroom grain spawn and ready to grow kits located in South East Queensland. We’re passionate about fungi and we love sharing our obsession with the world.

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