Specially modified jars are used for the production of grain spawn and liquid culture. Modified mason jars are used to sterilise media in preparation to be inoculated with a live mycelium culture. The jars need to be sealed to maintain the media free from contamination however fresh air exchange is required for the healthy growth of mycelium. The lids are modified to feature a filter for fresh air exchange and a self-healing injection port for easy injection and extraction of liquid cultures.
With these reusable jars you can easily create you own grain spawn or liquid culture with minimal risk of contamination.
What You'll Need
For this tutorial you will need glass jars. I recommend using 500 ml jars. You may use the size of your preference. Plastic lids are fine as long as they can withstand the conditions within the pressure cooker. Plastic lids will not rust and have a longer lifespan.
Step 1. Drill Holes In The Lid
Make 2 holes in each lid. 1 hole in the centre for fresh air exchange and one near the edge for the injection port. Widen the hole gradually to ensure that the fixings fit tightly in each hole before continuing.
Step 2. Prepare Expoxy
Prepare epoxy according to safety directions. Make sure to use gloves as this can be messy.
Step 3. Attach Filters & Injection Ports
Working quickly attach syringe filters to the centre holes and injection ports to the outer holes in each lid.
Micro pore tape is an excellent filter material on grain spawn jars due to the increased fresh air exchange.
Step 4. Allow Epoxy To Cure
Allow the epoxy to fully cure before using grain spawn & liquid culture jars.