How To Make
Grain Spawn
& Liquid Culture Jars

Step By Step Guide To Making Spawn & Liquid Culture Jars

Watch Our Video Guide Below

What Are Grain Spawn & Liquid Culture Jars?

Grain spawn and liquid culture jars are used to sterilise media in preparation to be inoculated with a live mycelium culture. The lids are modified to feature a filter for fresh air exchange and a self-healing injection port for easy injection and extraction of liquid cultures.

With these reusable jars you can easily create you own grain spawn or liquid culture with minimal risk of contamination. 

Grain Spawn & Liquid Culture Jars

What You'll Need

For this tutorial you will need glass jars. I recommend using 500 ml jars. You may use the size of your preference. Plastic lids are fine as long as they can withstand the conditions within the pressure cooker. Plastic lids will not rust and have a longer lifespan. 

  • Glass Jars
  • Step Drill Bit
  • Plastic Lids
  • PTFE Syringe Filters .22 micron (Hydrophobic)
  • Injection Ports
  • Expoxy
  • Tooth Picks
  • Gloves
  • Micro Pore Tape

Step 1. Drill Holes In The Lid

Make 2 holes in each lid. 1 hole in the centre for fresh air exchange  and one near the edge for the injection port. Ensure that the fixings fit in each hole before continuing.

Step 2. Prepare Expoxy

Prepare expoxy according to safety directions. Make sure to use gloves as this will be a messy procedure.

Step 3. Attach Filters & Injection Ports

Working quickly attach syringe filters to the centre holes and injection ports to the outer holes in each lid. Syringe filters should be used for liquid culture jars to reduce the likelihood of contamination.

Micro pore tape is an excellent filter material on grain spawn jars due to the increased fresh air exchange.

Step 4. Allow Epoxy To Cure

Allow the epoxy to fully cure before using grain spawn & liquid culture jars.

Are You Ready To Make Grain Spawn?

What is grain spawn? Why is it used? How is it produced? We answer these questions and more. Learn to make your own grain spawn at home with our video tutorial.

Learn How To Make Liquid Culture

Learn how to make liquid culture in our video tutorial and step-by-step guide. We show you how to make liquid culture following a few simple to follow principles and how to craft your own low-cost reusable liquid culture and spawn jars.

Who We Are

I’m Luke and I’m mad about mushrooms. I operate a small scale family run business located 40 minutes west of Brisbane. We’re passionate about fungi and we look forwarding to sharing our experiences with you.

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